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doi: 10.1016/j.bbrc.2013.05.089 113 KellerCCHittnerJBNtiBKWeinbergJBKremsnerPGPerkinsDJ

After the wash, the mitochondrial pellets were resuspended in an appropriate volume of sucrose buffer plus 1 PBS with 2% Triton X-100 plus protease and phosphatase inhibitors and stored at 80 C until further analysis

Here are the detailed recommendations based on clinical studies

Many experiments and studies have been conducted to examine the effectiveness of PEA in various neuropathic pain syndromes, yielding valuable insights into its clinical applicability [52]

10.1038/s41419-023-06206-z 12 LiW.CaoT.LuoC.CaiJ.ZhouX.XiaoX.et al (2020)